Resistance of soybean trypsin inhibitor (Kunitz) to denaturation by guanidinium chloride.
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منابع مشابه
Resistance of soybean trypsin inhibitor (Kunitz) to denaturation by guanidinium chloride.
Viscosity measurements in 6 M guanidinium chloride (Gdm.Cl) (pH 5.2, 25”) suggest that Kunitz soybean trypsin inhibitor (STI) undergoes a slow unfolding which requires over 2 weeks to reach completion. The reduced viscosity increased during this time from an initial value of 3.5 ml/g to a final value of 16 to 17 ml/g. At pH 7 and 25”, over 4 weeks were required to reach the same final state. Ge...
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Soybean (Glycine max (L.) Merr.) has emerged as one of the most economical and nutritious foods. Kunitz Soybean Trypsin Inhibitor (KSTI) and Bowman -Birk Inhibitor (BBI) are the two major trypsin inhibitors in soybeans. The ingested soybean trypsin inhibitors cause growth depression as demonstrated in different animal species and human. The main part of the present study was devoted to isolatio...
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Five trypsin and cu-chymotrypsin inhibitors which have low molecular weights (ranging from 6800 to 8600) and are present in soybean seeds of the Tracy variety have been isolated and purified, and single crystals which give x-ray diffraction data beyond 3-A spacings have been obtained from one of them. The trypsin inhibitor crystallizes in a monoclinic unit cell of symmetry P2, and dimensions a ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1977
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)63337-5